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KMID : 1123920120260040511
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Korean Journal of Oriental Physiology and Pathology
2012 Volume.26 No. 4 p.511 ~ p.518
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Effect of Vigna angularis on Toll-like Receptor Activation and Pro-inflammatory Cytokine Production
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Kim Mi-Hwa
Jeoung See-Hwa
Lee Seung-Woong
Kim Hyun-Kyu
Park Chan-Sun
Jeon Byung-Hoon
Oh Hyun-Mi
Rho Mun-Chual
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Abstract
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The mechanisms of Toll-like receptor (TLR) signaling have been the focus of extensive studies because TLRs are the target of therapeutic intervention on multiple diseases. In this study, we investigated the inhibitory potential of Vigna angularis (azuki bean) on the TLR signaling. The effect of Vigna angularis extract (JSD) on TLR activation was investigated by assessing NF-κB and AP-1 inducible secreted embryonic alkaline phosphatase (SEAP) activity. JSD significantly inhibited SEAP activity induced by poly I:C (TLR3 ligand) and poly I (TLR7 ligand) in a dose-dependent manner at concentration below 100 μg/ml with no sign of cytotoxicity. Pretreatment of JSD markedly suppressed mRNA expressions of pro-inflammatory cytokines and adhesive molecules such as TNF-α, IL-6, RANTES, MCP-1 and ICAM-1 induced by TLR ligands. It also diminished the phosphorylation of IκB kinase and IκB, and followed by Iκ B-mediated nuclear translocation of p50, p65, and phosphorylation of p38, JNK, and IRF signaling pathway. In conclusion, our results suggest that Vigna angularis has inhibitory activity on TLR-3 and -7 signaling and it can be further developed as a remedy in curing TLR-related multiple diseases.
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KEYWORD
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Toll-like receptor, Vigna angularis, NF-κB, TNF-α, IL-6
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